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逆行荧光染料标记允许有针对性的外单细胞记录技术鉴定神经元<em>在体内</em
JoVE Revista
Neurociencias
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JoVE Revista Neurociencias
Retrograde Fluorescent Labeling Allows for Targeted Extracellular Single-unit Recording from Identified Neurons In vivo
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逆行荧光染料标记允许有针对性的外单细胞记录技术鉴定神经元<em>在体内</em

DOI:
10.3791/3921-v

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12:32 min

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June 26, 2013

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, , ,
1Department of Biology,Washington University in St. Louis , 2Division of Biological Science, Graduate School of Science,Nagoya University, 3Department of Psychiatry,Washington University in St. Louis

Capítulos

  • 00:05Título
  • 02:26Pre-surgical Preparation
  • 04:08Surgery
  • 06:10Retrograde Labeling and Visualization of the Axon of Interest
  • 08:27Extracellular Activity Recording
  • 10:58Representative Results
  • 11:43Conclusion

Summary

Traducción Automática

Traducción Automática

逆行运输的荧光染料标记的基于解剖突起的神经元的一个子种群。可以在视觉对象标记的轴突<em>在体内</em>，允许确定的轴突外记录。这种技术有利于记录神经元不能被标记通过遗传操作或难以分离使用'盲人'<em>在体内</em>办法。

Tags

Retrograde Fluorescent LabelingTargeted Extracellular Single-unit RecordingIdentified NeuronsIn Vivo Electrophysiological RecordingsNeural CircuitsCellular IdentityIntracellular DyeJuxtacellular IontophoresisLoose-patch IontophoresisFluorescent ProteinsVisually-guided Single-cell ElectrophysiologyGenetic ToolsPromoterProjection PatternsViral VectorsTrans-synaptic SpecificityPre-visualizationTarget Neuron

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