Single-cell Analysis of <em>Bacillus subtilis</em> Biofilms Using Fluorescence Microscopy and Flow Cytometry

Juan C. Garcia-Betancur; Ana Yepes; Johannes Schneider; Daniel Lopez

doi:10.3791/3796

Journal

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Inmunología y contagio

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Tek hücreli Analizi Bacillus subtilis Biyofilmler

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Inmunología y contagio

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Single-cell Analysis of Bacillus subtilis Biofilms Using Fluorescence Microscopy and Flow Cytometry

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Tek hücreli Analizi Bacillus subtilis Biyofilmler

20,176 Views

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13:28 min

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February 15, 2012

DOI:

10.3791/3796-v

DOI:

10.3791/3796-v

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13:28 min

February 15, 2012

•

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Juan C. Garcia-Betancur¹, Ana Yepes¹, Johannes Schneider¹, Daniel Lopez¹

¹Institute for Molecular Infection Biology (IMIB),University of Würzburg

Summary

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Mikrobiyal biyofilmler genellikle özel hücreler farklı subpopülasyonunun oluşturduğu edilir. Bu alt popülasyonlarının Tek hücreli analizi floresan gazetecilere kullanımını gerektirir. Burada birkaç subpopulationswithin görselleştirmek ve izlemek için bir protokol tarif<em> B. subtilis</em> Floresan mikroskobu ve akım sitometri kullanarak biyofilm.

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Garcia-Betancur, J. C., Yepes, A., Schneider, J., Lopez, D. Single-cell Analysis of Bacillus subtilis Biofilms Using Fluorescence Microscopy and Flow Cytometry. J. Vis. Exp. (60), e3796, doi:10.3791/3796 (2012).

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