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Single-cell Analysis of Bacillus subtilis Biofilms Using Fluorescence Microscopy and Flow Cytometry
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JoVE Revista Inmunología y contagio
Single-cell Analysis of Bacillus subtilis Biofilms Using Fluorescence Microscopy and Flow Cytometry

Single-cell Analysis of Bacillus subtilis Biofilms Using Fluorescence Microscopy and Flow Cytometry

20,154 Views

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13:28 min

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February 15, 2012

DOI:

10.3791/3796-v

DOI:
10.3791/3796-v

•

13:28 min
February 15, 2012

•

3 Views
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1Institute for Molecular Infection Biology (IMIB),University of Würzburg

Tags

Single-cell AnalysisBacillus SubtilisBiofilmsFluorescence MicroscopyFlow CytometryExtracellular MatrixProteinsExopolysaccharidesSpecialized CellsSpatial OrganizationTemporal OrganizationMatrix ProducersFluorescent ReportersSurfactin

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Garcia-Betancur, J. C., Yepes, A., Schneider, J., Lopez, D. Single-cell Analysis of Bacillus subtilis Biofilms Using Fluorescence Microscopy and Flow Cytometry. J. Vis. Exp. (60), e3796, doi:10.3791/3796 (2012).

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