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Боковая диффузия и экзоцитоз мембранных белков в культивируемых нейронов оценивали по флуоресценции и флуоресценции восстановления потерь фотообесцвечивания
This content is Free Access.
Capítulos
- 00:05Título
- 01:33Cell Culture, Viral Transduction, and Protein Expression
- 02:16FRAP-FLIP Live Cell Imaging: Equipment Set-up and Image Capture Parameters
- 05:48FRAP-FLIP Live Cell Imaging: Image Acquisition
- 07:09Data Analysis
- 09:25Representative FRAP-FLIP Results
- 11:34Conclusion
Tags
Lateral DiffusionExocytosisMembrane ProteinsCultured NeuronsFluorescence RecoveryFluorescence-loss PhotobleachingTraffickingReceptorsIon ChannelsSynaptic ProteinsSuper-ecliptic PHluorinLive Cell ImagingPlasma Membrane ProteinsProtonationPhoton AbsorptionFluorescence EmissionIntracellular Trafficking EventsLow PH CompartmentsNeutral PH Extracellular EnvironmentPhotodamagePhotobleachingFRAP (fluorescence Recovery After Photobleaching)Protein Dynamics
