Journal
/
Inmunología y contagio
/
Locked Nucleic Acid Flowcytometri-fluorescens In situ Hybridisering (LNA flow-FISH): En metode til bakteriel små RNA Detection
JoVE Revista
Inmunología y contagio
This content is Free Access.
JoVE Revista Inmunología y contagio
Locked Nucleic Acid Flow Cytometry-fluorescence in situ Hybridization (LNA flow-FISH): a Method for Bacterial Small RNA Detection

Locked Nucleic Acid Flowcytometri-fluorescens In situ Hybridisering (LNA flow-FISH): En metode til bakteriel små RNA Detection

DOI:
10.3791/3655-v

•

09:45 min

•

January 10, 2012

•

,
1Center for Bio/Molecular Science and Engineering,Naval Research Laboratory

Capítulos

  • 00:05Título
  • 01:25LNA Probes and Experimental Design
  • 02:31Fixation
  • 03:36Diethypyrocarbonate (DEPC) Treatment and Permeabilization
  • 04:53Hybridization and Post-hybridization Washes
  • 05:47Blocking and Staining
  • 07:59Representative Cell Aggregation and LNA flow-FISH Data
  • 09:15Conclusion

Summary

Traducción Automática

Traducción Automática

En roman high-throughput Metoden er beskrevet som gør det muligt at afsløre og relative kvantificering af små RNA og mRNA udtryk fra enkelt bakterieceller ved hjælp af Locked Nucleic Acid sonder og flowcytometri-fluorescens<em> In situ</em> Hybridisering.

Tags

Locked Nucleic AcidFlow CytometryFluorescence In Situ HybridizationFISHNucleic Acid SequencesFlow-FISHLysate-based Nucleic Acid Detection MethodsStatistical AnalysisVariance AnalysisTelomere Length DeterminationCellular IdentificationGene ExpressionViral MultiplicationBacterial Community AnalysisDNA Oligonucleotide ProbesNucleic Acid AnalogsSensitivitySpecificityMelting Temperatures (Tm)Locked Nucleic Acid (LNA) Probes

Article

Embed

Añadir a la lista de reproducción

Usage Statistics

Videos relacionados

Combination of Adhesive-tape-based Sampling and Fluorescence in situ Hybridization for Rapid Detection of Salmonella on Fresh Produce

Transfection and Mutagenesis of Target Genes in Mosquito Cells by Locked Nucleic Acid-modified Oligonucleotides

'Bioluminescent' Reporter Phage for the Detection of Category A Bacterial Pathogens

Hybridisering<em> In situ</em> Af spytkirtler, æggestokke og embryoner af Vector Myg

Fluorescent<em> In situ</em> Hybridisering på mitotiske kromosomer af myg

Bakteriel kunstige kromosomer: En funktionel Genomics værktøj for Studiet af Positiv-RNA-vira

Hurtig, sikker og simpel manuel sengelamper nukleinsyre udvinding til påvisning af Virus i blodprøver

Kvantitativ fluorescens in situ-hybridisering (fisk) og immunofluorescens (IF) af specifikke genprodukter i KSHV-inficerede celler

Modellering Hepatitis B Virus Infektion i ikke-hepatisk 293T-NE-3NRs celler

En ikke-kodende lille RNA-mikrofon bidrager til virulens i ydre membranproteiner i Salmonella enteritidis

Read Article