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Capítulos
- 00:03Título
- 03:00Step 1 Making horizontal hippocampal/cortical slices from young mouse brain
- 05:57Step 2 Preparation of the staining chamber
- 08:48Step 3 Staining of slices
- 10:21Step 4 Non-neuronal dyes and older tissue
- 12:02Step 5 Attaching slice to recording chamber
- 13:36Step 6 Imaging
- 15:58Conclusion
Spontan aktivitet for utvikling av nevrale nettverk kan måles ved hjelp av AM-ester former for kalsium-sensitive indikator fargestoffer. Endringer i intracellulær kalsium, noe som indikerer neuronal aktivering, oppdages som forbigående endringer i indikator fluorescens med ett-eller to-foton imaging. Denne protokollen kan tilpasses en rekke developmentally-avhengige nevrale nettverk<em> In vitro</em>.
Tags
Functional Calcium ImagingDeveloping Cortical NetworksSynchronized Network ActivitySpontaneous ActivityDepolarizationVoltage-gated Calcium ChannelsSynchronized Electrical ActivityField ElectrodesPatch-clamp ElectrophysiologyCalcium-dependent Fluorescent Indicator DyesSpatial ResolutionTemporal Sampling
