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Spektral konfokal afbildning af Fluorescerende mærkede nikotinreceptorer i Knock-i mus med kronisk Nikotin Administration
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Capítulos
- 00:05Título
- 01:45Intracardiac Perfusion and Brain Tissue Sectioning
- 03:15Imaging Fluorescent nAChRs using Spectral Confocal Microscopy
- 04:16Linear Unmixing of Spectral Confocal Images and Image Analysis
- 06:23Quantification and Localization of YFP-tagged alpha4 nAChRs in Mouse Brain using Spectral Confocal Microscopy
- 08:10Conclusion
Vi har udviklet en ny teknik til at kvantificere nicotinacetylcholinreceptorneurotransmission ændringer inden for subcellulære regioner af specifikke undertyper af CNS-neuroner til bedre at forstå mekanismerne i nikotintrangen ved hjælp af en kombination af metoder, herunder fluorescerende protein tagging af receptoren ved hjælp af knock-in tilgang og spektral konfokal billeddannelse.
Tags
Spectral Confocal ImagingFluorescently Tagged Nicotinic ReceptorsKnock-in MiceChronic Nicotine AdministrationLigand-gated Ion ChannelsCentral Nervous System (CNS)Addiction To NicotineIon Channel DistributionChronic Nicotine ExposureNicotinic Acetylcholine Receptors (nAChRs)Glutamatergic GluN1GluA1 ChannelsSensitization To Addictive DrugsMap And Quantify DistributionExpression PatternsSpecific Ion ChannelsMechanisms Of AddictionBrain Region-specific EffectsRadioactive LigandsLow Spatial ResolutionLigand-gated Ion Channels In NeuronsGenetically Encoded Fluorescent Reporters
