Journal
/
Inmunología y contagio
/
Isolamento de RNA Pseudomonas aeruginosa Colonizar o trato gastrointestinal Murino
JoVE Revista
Inmunología y contagio
This content is Free Access.
JoVE Revista Inmunología y contagio
RNA Isolation of Pseudomonas aeruginosa Colonizing the Murine Gastrointestinal Tract

Isolamento de RNA Pseudomonas aeruginosa Colonizar o trato gastrointestinal Murino

18,833 Views

•

08:52 min

•

September 28, 2011

DOI:

10.3791/3293-v

DOI:
10.3791/3293-v

•

08:52 min
September 28, 2011

•

6 Views
, , ,
1Department of Pediatrics,University of Texas Southwestern Medical Center , 2Channing Laboratory, Department of Medicine, Brigham and Women’s Hospital,Harvard Medical School, 3Department of Pediatrics and Microbiology,University of Texas Southwestern Medical Center

Summary

Automatically generated

Um método confiável para o isolamento do RNA<em> Pseudomonas aeruginosa</em> Recuperou de cecums murino é descrito. O RNA recuperado é de quantidade e qualidade suficientes para qPCR subseqüentes, perfis de transcrição, RNA e experimentos Seq. Esta técnica pode ser adaptada para o isolamento de RNA outros micróbios intestinais.

Explore More Videos

RNA IsolationPseudomonas AeruginosaGastrointestinal TractColonizationImmune SystemsLeukemiaBurn WoundsOrgan TransplantsBloodstream InfectionsTranscription ProfilingMRNAGene ExpressionImmune StatusIsolationHigh Quality MRNAEnzymesDebrisFood ResiduesParticulate MatterGI ColonizationNeutropenia-induced DisseminationMice Euthanization

Read Article

Cite this Article

Lopez-Medina, E., Neubauer, M. M., Pier, G. B., Koh, A. Y. RNA Isolation of Pseudomonas aeruginosa Colonizing the Murine Gastrointestinal Tract. J. Vis. Exp. (55), e3293, doi:10.3791/3293 (2011).

Download .ris file

Copy

Share Video

.

Copy