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Preparing E18 Cortical Rat Neurons for Compartmentalization in a Microfluidic Device
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JoVE Revista Biología
Preparing E18 Cortical Rat Neurons for Compartmentalization in a Microfluidic Device

Preparing E18 Cortical Rat Neurons for Compartmentalization in a Microfluidic Device

19,398 Views

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12:00 min

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October 01, 2007

DOI:

10.3791/305-v

DOI:
10.3791/305-v

•

12:00 min
October 01, 2007

•

14 Views
, , , , ,
1Department of Biomedical Engineering,University of California, Irvine (UCI), 2Stem Cell Research Center,University of California, Irvine (UCI), 3Institute for Brain Aging and Dementia,University of California, Irvine (UCI)

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In this video we demonstrate the preparation of E18 Cortical Rat Neurons.

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E18 Cortical Rat NeuronsMicrofluidic DevicePreparationDissociationViabilityDissection BufferTrypsinIncubationDMEMFBSCentrifugationNeural Basal Media (NBM)GlutamaxTrituratingCell SuspensionNylon Ce

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Harris, J., Lee, H., Tu, C. T., Cribbs, D., Cotman, C., Jeon, N. L. Preparing E18 Cortical Rat Neurons for Compartmentalization in a Microfluidic Device. J. Vis. Exp. (8), e305, doi:10.3791/305 (2007).

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