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A Real-time Electrical Impedance Based Technique to Measure Invasion of Endothelial Cell Monolayer by Cancer Cells
 

A Real-time Electrical Impedance Based Technique to Measure Invasion of Endothelial Cell Monolayer by Cancer Cells

Article DOI: 10.3791/2792-v • 08:51 min • April 1st, 2011
April 1st, 2011 •
1, 1
1Lombardi Comprehensive Cancer Center, Georgetown University

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This article describes an in vitro technique for monitoring cancer cells invading through a monolayer of endothelial cells. The data is acquired in real-time as a function of changes in impedance on the surface of electrodes at the well bottom.

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Real-time Electrical Impedance Technique Measure Invasion Endothelial Cell Monolayer Cancer Cells Metastatic Dissemination Basement Membrane Degradation Tumor Cell Attachment Vascular Endothelium Endothelial Junction Retraction Invasion And Migration Circulatory System Adherence To Capillary Walls Extravasation To Surrounding Tissue Metastatic Tumors Formation Tumor Cell-endothelial Cell Interaction In Vitro Replication Monolayer Of Human Umbilical Vein Endothelial Cells (HUVEC) Cancer Cell Challenge Electron Microscopy Phase-contrast Microscopy In Vivo Metastatic Process Representation Electrical Impedance Fluctuations Measurement XCELLigence Instrument Roche Manufacturing
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