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Maya İzleme Mitophagy Floresan Mikroskopi Testi Saccharomyces cerevisiae
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Biología
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JoVE Revista Biología
A Fluorescence Microscopy Assay for Monitoring Mitophagy in the Yeast Saccharomyces cerevisiae
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Maya İzleme Mitophagy Floresan Mikroskopi Testi Saccharomyces cerevisiae

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10:27 min

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July 18, 2011

DOI:

10.3791/2779-v

DOI:
10.3791/2779-v

•

10:27 min
July 18, 2011

•

4 Views
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1Department of Biochemistry and Molecular Biology, School of Biomedical Sciences,Monash University

Summary

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Bozulma ve geri dönüşüm için maya vakuol asidik lümen organelleri teslim izlemek için sağlam bir yaklaşım açıklanmıştır. Bu yöntem, genetik olarak kodlanmış çift emisyon floresan pH biyosensör ve floresan mikroskobu kullanarak tek tek hücrelerin görselleştirme hedef organellerin özel etiketleme dayanmaktadır.

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Cite this Article

Mijaljica, D., Prescott, M., Devenish, R. J. A Fluorescence Microscopy Assay for Monitoring Mitophagy in the Yeast Saccharomyces cerevisiae. J. Vis. Exp. (53), e2779, doi:10.3791/2779 (2011).

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