Journal
/
Biología
/
Isolamento de Células-Tronco do rato das Glândulas Salivares
JoVE Revista
Biología
This content is Free Access.
JoVE Revista Biología
Isolation of Mouse Salivary Gland Stem Cells

Isolamento de Células-Tronco do rato das Glândulas Salivares

23,207 Views

•

09:52 min

•

February 08, 2011

DOI:

10.3791/2484-v

DOI:
10.3791/2484-v

•

09:52 min
February 08, 2011

•

13 Views
, , , ,
1Department of Cell Biology,University Medical Center Groningen, University of Groningen, 2Department of Radiation Oncology,University Medical Center Groningen, University of Groningen

Summary

Automatically generated

Um protocolo otimizado para o isolamento de células-tronco a partir da glândula salivar do mouse é descrito. O método emprega digestão enzimática e mecânica, e permite o isolamento de salispheres contendo células com características de células-tronco.

Explore More Videos

IsolationMouse Salivary Gland Stem CellsSalivary GlandsCell TypesProductionExcretionSalivaAcinar CellsProtein ProductionMucous ProductionDuctal CellsEpithelial-type MorphologySaliva CompositionDiseasesSjögren’s SyndromeRadiotherapy TreatmentXerostomiaDry MouthDental CariesRegenerative CapacityStem Cell-like Populations

Read Article

Cite this Article

Pringle, S., Nanduri, L. S. Y., Marianne, v. d. Z., Ronald, v. O., Coppes, R. P. Isolation of Mouse Salivary Gland Stem Cells. J. Vis. Exp. (48), e2484, doi:10.3791/2484 (2011).

Download .ris file

Copy

Share Video

.

Copy