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のための負の選択マーカーの開発赤痢アメーバ</em
JoVE Revista
Inmunología y contagio
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JoVE Revista Inmunología y contagio
Development of a Negative Selectable Marker for Entamoeba histolytica
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のための負の選択マーカーの開発赤痢アメーバ</em

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06:05 min

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December 12, 2010

DOI:

10.3791/2410-v

DOI:
10.3791/2410-v

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06:05 min
December 12, 2010

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1Division of Infectious Disease and International Health,University of Virginia Health System

Tags

Negative Selectable MarkerEntamoeba HistolyticaAmebiasisGene ExpressionTransfectionPlasmidsReverse GeneticsHomologous RecombinationEpisomal Plasmid DNABackground Of Exogenous DNAGenomic Integration EventNegative Selection SystemYeast Cytosine Deaminase And Uracil Phosphoribosyl Transferase Chimera (FCU1)Prodrug 5-fluorocytosine (5-FC)5-fluorouracil5-fluorouridine-5′-monophosphate

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Abhyankar, M. M., Haviland, S. M., Gilchrist, C. A., Petri, Jr., W. A. Development of a Negative Selectable Marker for Entamoeba histolytica. J. Vis. Exp. (46), e2410, doi:10.3791/2410 (2010).

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