Development of a Negative Selectable Marker for <em>Entamoeba histolytica</em>

Mayuresh M Abhyankar; Sarah M Haviland; Carol A Gilchrist; William A Petri, Jr.

doi:10.3791/2410

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Inmunología y contagio

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एक नकारात्मक के लिए चयन मार्कर का विकास एटामोइबा हिस्टोलिटिका</em

JoVE Revista
Inmunología y contagio

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JoVE Revista Inmunología y contagio

Development of a Negative Selectable Marker for Entamoeba histolytica

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एक नकारात्मक के लिए चयन मार्कर का विकास एटामोइबा हिस्टोलिटिका</em

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December 12, 2010

DOI:

10.3791/2410-v

DOI:

10.3791/2410-v

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06:05 min

December 12, 2010

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Mayuresh M Abhyankar, Sarah M Haviland, Carol A Gilchrist, William A Petri, Jr.

¹Division of Infectious Disease and International Health,University of Virginia Health System

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Negative Selectable Marker Entamoeba Histolytica Amebiasis Gene Expression Transfection Plasmids Reverse Genetics Homologous Recombination Episomal Plasmid DNA Background Of Exogenous DNA Genomic Integration Event Negative Selection System Yeast Cytosine Deaminase And Uracil Phosphoribosyl Transferase Chimera (FCU1)Prodrug 5-fluorocytosine (5-FC)5-fluorouracil 5-fluorouridine-5′-monophosphate

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Abhyankar, M. M., Haviland, S. M., Gilchrist, C. A., Petri, Jr., W. A. Development of a Negative Selectable Marker for Entamoeba histolytica. J. Vis. Exp. (46), e2410, doi:10.3791/2410 (2010).

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एक नकारात्मक के लिए चयन मार्कर का विकास एटामोइबा हिस्टोलिटिका</em

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