Development of a Negative Selectable Marker for <em>Entamoeba histolytica</em>

Mayuresh M Abhyankar; Sarah M Haviland; Carol A Gilchrist; William A Petri, Jr.

doi:10.3791/2410

Journal

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Inmunología y contagio

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وضع علامة سلبية لإختيار a المتحولة الحالة للنسج</em

JoVE Revista
Inmunología y contagio

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JoVE Revista Inmunología y contagio

Development of a Negative Selectable Marker for Entamoeba histolytica

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وضع علامة سلبية لإختيار a المتحولة الحالة للنسج</em

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06:05 min

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December 12, 2010

DOI:

10.3791/2410-v

DOI:

10.3791/2410-v

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06:05 min

December 12, 2010

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Mayuresh M Abhyankar, Sarah M Haviland, Carol A Gilchrist, William A Petri, Jr.

¹Division of Infectious Disease and International Health,University of Virginia Health System

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Negative Selectable Marker Entamoeba Histolytica Amebiasis Gene Expression Transfection Plasmids Reverse Genetics Homologous Recombination Episomal Plasmid DNA Background Of Exogenous DNA Genomic Integration Event Negative Selection System Yeast Cytosine Deaminase And Uracil Phosphoribosyl Transferase Chimera (FCU1)Prodrug 5-fluorocytosine (5-FC)5-fluorouracil 5-fluorouridine-5′-monophosphate

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Abhyankar, M. M., Haviland, S. M., Gilchrist, C. A., Petri, Jr., W. A. Development of a Negative Selectable Marker for Entamoeba histolytica. J. Vis. Exp. (46), e2410, doi:10.3791/2410 (2010).

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