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重组逆转录病毒的生产和B细胞的感染
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Inmunología y contagio
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JoVE Revista Inmunología y contagio
Recombinant Retroviral Production and Infection of B Cells
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重组逆转录病毒的生产和B细胞的感染

13,966 Views

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09:19 min

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February 18, 2011

DOI:

10.3791/2371-v

DOI:
10.3791/2371-v

•

09:19 min
February 18, 2011

•

5 Views
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1Department of Microbiology and Immunology,Columbia University College of Physicians and Surgeons, 2Herbert Irving Comprehensive Cancer Center,Columbia University

Summary

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一个有效率的制度中的一个基因的结构和功能分析<em>体外</em>脾B淋巴细胞的文化描述。这种方法需要利用一个辅助的自由，ecotrophic包装细胞系的重组逆转录病毒的生产。稳定，遗传兴趣在初级淋巴细胞基因的表达，是实现领导在接受类开关重组的B细胞表面抗体的生成。

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Cite this Article

Keim, C., Grinstein, V., Basu, U. Recombinant Retroviral Production and Infection of B Cells. J. Vis. Exp. (48), e2371, doi:10.3791/2371 (2011).

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