Se requiere una suscripción a JoVE para ver este contenido. Inicie sesión o comience su prueba gratuita.
Capítulos
- 00:00Título
- 01:13Habituating Animals
- 01:29Eliciting Target-Specific Effector CTLs by Immunization (Day 0)
- 03:03Program Operant Test Session
- 03:16Harvesting Target Cells (Day 8)
- 05:32Treatment and Labeling of Target Cells
- 06:33Results: Operant Sensation Seeking Behavior
- 07:39Adoptive Transfer and Subsequent Harvest of Labeled Cells
- 08:06Conclusion
- 08:20Flow Cytometry and Data Analysis
- 08:36CFSE Profile Demonstrating Cell-Specific Killing in BALB/c Mice
- 09:34Conclusion
Tags
Antigen Specific In Vivo Killing AssayCFSE Labeled Target CellsCarboxyfluorescein Diacetate Succinimidyl EsterIn Vivo InvestigationCell LabelingAdoptive TransferSurvivalKillingEpitope SpecificCD8+ T Cell CloneResponse QualityCytokine ProductionFunctional ExhaustionTCR SpecificitiesCell Mediated ImmunityResponding T CellsPeptide Specific T Cell ClonesBALB/c Mice
