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Mutagenesis and Functional Analysis of Ion Channels Heterologously Expressed in Mammalian Cells
JoVE Revista
Biología
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JoVE Revista Biología
Mutagenesis and Functional Analysis of Ion Channels Heterologously Expressed in Mammalian Cells

Mutagenesis and Functional Analysis of Ion Channels Heterologously Expressed in Mammalian Cells

DOI:
10.3791/2189-v

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15:28 min

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October 01, 2010

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, ,
1Clayton Foundation Laboratories for Peptide Biology,Salk Institute for Biological Studies

Capítulos

  • 00:00Título
  • 01:06Introduction
  • 01:44Site Directed Mutagenesis and Preparation of Plasmid DNA for Transfection
  • 03:09Plating of Cells, Transient Transfection, and Imaging of Transfected Cells
  • 05:00Whole-cell Patch Clamping
  • 12:57Representative Analysis
  • 14:42Conclusion

Summary

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Traducción Automática

We will demonstrate how to study the effect of a single point mutation on the function of an ion channel.

Tags

MutagenesisFunctional AnalysisIon ChannelsHeterologously ExpressedMammalian CellsPoint MutationGIRK ChannelsG Protein-gated Inwardly Rectifying Potassium ChannelsExcitability Of NeuronsG Protein-coupled ReceptorsMuscarinic ReceptorAlcohol ActivationAmino Acid MutationCDNA SequencingTransfectionHEK293T CellsPatch-clamp ElectrophysiologyPotassium CurrentsL257W Mutation

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