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반대 미리 정의된 T 세포 수용체 Specificities와 Transnuclear 쥐 Toxoplasma gondii SCNT 비아 획득
JoVE Revista
Inmunología y contagio
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JoVE Revista Inmunología y contagio
Transnuclear Mice with Pre-defined T Cell Receptor Specificities Against Toxoplasma gondii Obtained Via SCNT
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반대 미리 정의된 T 세포 수용체 Specificities와 Transnuclear 쥐 Toxoplasma gondii SCNT 비아 획득

13,827 Views

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13:36 min

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September 30, 2010

DOI:

10.3791/2168-v

DOI:
10.3791/2168-v

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13:36 min
September 30, 2010

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10 Views
, , , , ,
1Whitehead Institute for Biomedical Research, 2Departments of Microbiology and Biological Sciences,National University of Singapore, 3Department of Biology,Massachusetts Institute of Technology

Tags

Transnuclear MicePre-defined T Cell Receptor SpecificitiesToxoplasma GondiiSCNTLymphocytesGenetic RecombinationAntigen-specific T Cell ReceptorT Cell DevelopmentT Cell FunctionLong-term CultureAntigen StimulationHigh AffinityRandom Genomic IntegrationExpression LevelKineticsEpigenetic ReprogrammingSomatic Cell Nuclear TransferEmbryonic Stem CellsMouse ModelsEndogenous LociDisease Models

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Kirak, O., Frickel, E., Grotenbreg, G. M., Suh, H., Jaenisch, R., Ploegh, H. L. Transnuclear Mice with Pre-defined T Cell Receptor Specificities Against Toxoplasma gondii Obtained Via SCNT. J. Vis. Exp. (43), e2168, doi:10.3791/2168 (2010).

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