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真核Polyribosome剖面分析
JoVE Revista
Biología
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JoVE Revista Biología
Eukaryotic Polyribosome Profile Analysis
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真核Polyribosome剖面分析

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09:16 min

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June 15, 2010

DOI:

10.3791/1948-v

DOI:
10.3791/1948-v

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09:16 min
June 15, 2010

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13 Views
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1Department of Molecular Genetics, Microbiology, and Immunology,University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School

Summary

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本文介绍了协议的真核细胞的核糖体翻译的提取。一旦提取，核糖体是由蔗糖梯度分馏分离到monosomes和多聚核糖体，允许不同的核糖体的人群进行分析。因此，这种方法是研究翻译调控的黄金标准。

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Cite this Article

Esposito, A. M., Mateyak, M., He, D., Lewis, M., Sasikumar, A. N., Hutton, J., Copeland, P. R., Kinzy, T. G. Eukaryotic Polyribosome Profile Analysis. J. Vis. Exp. (40), e1948, doi:10.3791/1948 (2010).

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