Journal
/
Biología
/
Monitoring Heart Function in Larval Drosophila melanogaster for Physiological Studies
JoVE Revista
Biología
This content is Free Access.
JoVE Revista Biología
Monitoring Heart Function in Larval Drosophila melanogaster for Physiological Studies

Monitoring Heart Function in Larval Drosophila melanogaster for Physiological Studies

DOI:
10.3791/1596-v

•

09:37 min

•

November 16, 2009

•

, , , ,
1Department of Biology,University of Kentucky, Lexington

Capítulos

  • 00:00Título
  • 00:29Introduction
  • 00:55Unrestrained Intact Larvae: The “Ant Farm” Method
  • 02:01Restrained Intact Larvae: The “Glue Method”
  • 03:52Larvae Dissection
  • 05:43Electrical Pacing
  • 07:21Field Potentials and Intracellular Recordings
  • 08:58Conclusion

Summary

Traducción Automática

Traducción Automática

We present various ways to monitor heart function in the larva of Drosophila for assessing questions dealing with the function of gap junctions, ion channel mutations, modulation of pacemaker activity and pharmacological studies.

Tags

MonitoringHeart FunctionLarval Drosophila MelanogasterPhysiological StudiesCardiac FunctionHeart RateUnrestrained LarvaeRestrained LarvaeEnvironment ControlDissected LarvaeInternal Organs RemovalBathe Heart In CompoundsExposed HeartMembrane PotentialsIonic CurrentsMyocytesElectrical ConductionHeart TubeAdvantagesDisadvantagesFuture ExperimentsLarval Heart PreparationDrosophila Skeletal NMJIntracellular Calcium RegulationCellular FunctionAction PotentialsMyocytesSpeciesIonic DysfunctionsGenes Responsible For Diseases In Mammals

Article

Embed

Añadir a la lista de reproducción

Usage Statistics

Videos relacionados

Dissection of Larval CNS in Drosophila Melanogaster

High-Resolution Video Tracking of Locomotion in Adult Drosophila Melanogaster

Drosophila Larval NMJ Dissection

Drosophila Larval NMJ Immunohistochemistry

Visually Mediated Odor Tracking During Flight in Drosophila

Visualizing the Beating Heart in Drosophila

Preparation of embryos for Electron Microscopy of the Drosophila embryonic heart tube

Heart Dissection in Larval, Juvenile and Adult Zebrafish, Danio rerio

Measurement of Lifespan in Drosophila melanogaster

Drosophila Preparation and Longitudinal Imaging of Heart Function In Vivo Using Optical Coherence Microscopy (OCM)

Read Article