Intranasal Immunization with BCG in a Mouse Model
Published: May 31, 2024
Abstract
Source: Uranga, S. et al., Protective Efficacy and Pulmonary Immune Response Following Subcutaneous and Intranasal BCG Administration in Mice. J. Vis. Exp. (2016)
This video demonstrates intranasal BCG immunization in mice, activating both innate and adaptive immune responses, leading to the development of lung-resident memory T cells and effector B cells, thus enhancing protection against Mycobacterium infections.
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Preparation of Quantified Glycerol Stocks of Bacillus Calmette-Guerin (BCG) Danish and Mycobacterium tuberculosis H37Rv NOTE: All the protocols described were performed under bio-safety level-3 (BSL3) conditions. Thaw frozen glycerol stocks of BCG Danish or …
Divulgaciones
The authors have nothing to disclose.
Materials
| Middlebrook 7H9 broth | BD | 271310 | |
| Middlebrook ADC Enrichment | BD | 211887 | |
| Tween 80 | Scharlau | TW00800250 | |
| 3-mm diameter Glass Beads | Scharlau | 038-138003 | |
| Middlebrook 7H10 Agar | BD | 262710 | |
| 1-ml syringe 26GA 0.45×10 mm | BD | 301358 | |
| C tubes | Miltenyi Biotec | 130-093-237 | |
| M tubes | Miltenyi Biotec | 130-093-236 | |
| LACHRYMAL OLIVE LUER LOCK 0.60 x 30 mm. 23G x 1 1/4" | UNIMED | 27.134 | Used as trachea cannula for BAL |
| Albumin, from bovine serum | Sigma | A4503 |
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Intranasal Immunization with BCG in a Mouse Model. J. Vis. Exp. (Pending Publication), e22249, doi: (2024).