A Technique to Generate and Deliver Drug Aerosols Directly to Mouse Lungs
Published: May 31, 2024
Abstract
Source: Phillips, J. E. et al., Dry Powder and Nebulized Aerosol Inhalation of Pharmaceuticals Delivered to Mice Using a Nose-only Exposure System. J. Vis. Exp. (2017)
This video demonstrates the generation of drug aerosols and the targeted nose-only delivery of these aerosols to mice using an inhalation unit equipped with an aerosol generator. The mouse is restrained in a nose-only restrainer and connected to the inhalation unit, which delivers the drug directly into the mouse's nasal cavity. Subsequently, the drug passes through the trachea and enters the lungs.
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Formulation and Device Selection for the Generation of Pharmaceutical Aerosols NOTE: Formulation and device selection depend on the individual drug's physicochemical properties to be aerosolized. Nebulized liquid aerosols. Dissolve the drug in…
Representative Results
Figure 1: Inhalation unit with dry powder aerosol generator attached. The nebulizer used to generate aerosol from liquid formulations is shown on the right. 1) Wright dust feed, 2) cyclone, 3) syringe pump, 4) jet nebulizer inserted into pre-separator, 5) dilution air mixer. When delivering nebulized aerosols, the dust feed, and cyclone are replaced by the syringe pump, jet nebulizer/pre-sep…
Divulgaciones
The authors have nothing to disclose.
Materials
| Nose-only exposure inhalation unit | TSE systems | 700100-KNES-040-ss | Custom configurations available |
| DACO data acquisition system | TSE systems | 700400-PRO-C-D/1 | |
| MC One Jet Mill | Jetpharma | DEC MicroJet 10 | |
| Turbula Mixer | GlenMills Inc | T2F | |
| Micronized Lactose | DFE Pharma | Lactohale 200 | |
| Hydraulic press | Specac | GS15011 | |
| Cascade impactor filters | Pall Life Sciences | 7219 | Emfab filter |
| Absolute filters | Whatman | 10370302 | 5 cm diameter |
| Real time aerosol monitor Microdust Pro Monitor |
Casella | CEL-712 | |
| Ipratropium bromide | Spectrum Chemical | I1178 | pre-micronized |
| flexiVent FX1 system | scireq | FV-FXCS |
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A Technique to Generate and Deliver Drug Aerosols Directly to Mouse Lungs. J. Vis. Exp. (Pending Publication), e22246, doi: (2024).