Intrabronchial Instillation of Streptococcus pneumoniae in Immunocompetent Rodent Models

Published: October 31, 2023

Abstract

Source: Hoover, J. L., et al., A Robust Pneumonia Model in Immunocompetent Rodents to Evaluate Antibacterial Efficacy against S. pneumoniae, H. influenzae, K. pneumoniae, P. aeruginosa or A. baumannii. J. Vis. Exp. (2017)

This video demonstrates the establishment of lung infection in an immunocompetent rodent model via intubation and installation of bacterial suspensions into lung lobes. This model enables antibacterial efficacy studies in immunocompetent animals.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Anesthetize, Intubate, and Inoculate Animals NOTE: Specific pathogen-free (SPF) immunocompetent male Sprague-Dawley rats weighing 100 – 120 g are recommended. Provide all animals with food and water ad libitum and house them socially on wood chips or other absorbent bedding with s…

Representative Results

Figure 1: Nonsurgical Intratracheal Intubation. Tools are shown for intubating the rat (A) and the mouse (B). Once anesthetized, the animal should be positioned as shown in C, with the head to the right and tail to the left if the individual performing the technique is right-handed. Place the left forefinger gently on the throat …

Divulgaciones

The authors have nothing to disclose.

Materials

Guide Tool Intek Services Ltd GT01
1 mL TB syringe slip tip Becton Dickinson and Company 309659
PrecisionGlide Needle 25 G x 5/8 Becton Dickinson and Company 305122
Intramedic polyethylene tubing Clay Adams brand – Becton Dickinson and Company 427401 Plastic cannula for mouse
Animal feeding needle – straight 20 x 3" 2-1/4 Popper and Sons, Inc 7903 Used to create metal cannula for mice
75 TN 5.0 µL syringe 26s/2"/2 Hamilton 87930 HPLC glass injection syringe
Difco Nutrient Agar Becton Dickinson and Company 213000
Pyrex tube, culture 25×150 screwcap with rubber line Corning 9825-25 To autoclave/store metal cannulae
PrecisionGlide Needle 25 G x 5/8 Becton Dickinson and Company 305122
70% isopropyl alcohol Vi-Jon (Swan) NDC 0869-0810-43
Isoflurane, USP Piramal Healthcare NDC 66794-013-10
50 mL Polypropylene Conical Tube 30 x 115 mm Falcon, a Corning Brand 352070
75 TN 5.0 µL syringe 26s/2"/2 Hamilton 87930 HPLC glass injection syringe

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Intrabronchial Instillation of Streptococcus pneumoniae in Immunocompetent Rodent Models. J. Vis. Exp. (Pending Publication), e21758, doi: (2023).

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