Isolation of Lymphocytes from a Mouse Peyer's Patch
Published: July 31, 2023
Abstract
Source: Yazicioglu, Y. F., et al. Unraveling Key Players of Humoral Immunity: Advanced and Optimized Lymphocyte Isolation Protocol from Murine Peyer's Patches. J. Vis. Exp. (2018)
In this video, we demonstrate the isolation of lymphocytes from a harvested mouse Peyer's patch. The isolated lymphocytes can be used for further analysis.
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Surgical Excision and Tissue Preparation Steps: Surgical removal of the Small Intestine (SI) Euthanize mice using CO2 asphyxiation or any equivalent method approved by the institutional animal ethics committee. Transfer the mouse to a dedicated area for surgical excision. Place the back…
Divulgaciones
The authors have nothing to disclose.
Materials
| 6-well, 12-well, & 96-well plates | Falcon/Corning | 353046,353043/3596 | |
| 50 ml conical tubes | Falcon | 3520 | |
| 40 µm cell strainer | Falcon | 352340 | |
| 10 ml syringe-plunger | Exel INT | 26265 | |
| RPMI | Corning | 15-040-CV | |
| PBS | Corning | 21-040-CM | |
| FBS | Atlanta Biologicals | S11150 | |
| Orbital shaker | VWR | Model 200 | |
| Curved-end scissor | |||
| Fine Serrated Forceps | |||
| Small curved scissor |
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Isolation of Lymphocytes from a Mouse Peyer's Patch. J. Vis. Exp. (Pending Publication), e21518, doi: (2023).