Cellular Impedance-Based Analysis for Monitoring of Cellular Processes in Real-Time
Published: April 30, 2023
Abstract
Source: Labadie, T., et al., Monitoring Influenza Virus Survival Outside the Host Using Real-Time Cell Analysis. J. Vis. Exp. (2021)
In this video, we demonstrate the process of real-time monitoring of cellular events, including the proliferation of adherent cells, via a cellular impedance assay. Here, the adherent cells act as insulators, restricting the current flow between the electrodes and increasing the impedance.
Protocol
NOTE: During all experiments, keep the plates on non-electrostatic surfaces at all times, such as the paper wraps from the packaging. Follow section 1 below to determine the most appropriate concentration of cells to be seeded in the electronic microtiter plate (designed as E-Plate). 1. Determination of appropriate cell quantity for infecting cells Prepare MDCK (Madin-Darby Canine Kidney) cells in 75 cm2 flasks to obtain freshly split cells (approximatel…
Divulgaciones
The authors have nothing to disclose.
Materials
| E-Plate 16 (6 plates) | ACEA Biosciences, Inc | 5469830001 | E-plates are available in different packaging |
| PBS 1X | Life technologies (gibco) | 14040091 | |
| TPCK-Trypsin | Worthington | LS003740 | |
| xCELLigence Real-Time Cell Analysis Instrument S16 | ACEA Biosciences, Inc | 380601310 | The xCELLigence RTCA S16 instruments are available in different formats (16-well, 96-well, single or multi-plate) |
| 75 cm2 tissue culture flask | Falcon | 430641U | |
| MEM 1X | Life technologies (gibco) | 31095029 |
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Cellular Impedance-Based Analysis for Monitoring of Cellular Processes in Real-Time. J. Vis. Exp. (Pending Publication), e21323, doi: (2023).