Ultrasound-Guided Concanavalin A Injection Into Inferior Lacrimal Gland: A Technique to Inoculate Inferior Lacrimal Gland With Concanavalin A to Induce Dry Eye Disease in Rabbit Model

Published: April 30, 2023

Abstract

Source: Honkanen, R. A. et al. A Rabbit Model of Aqueous-Deficient Dry Eye Disease Induced by Concanavalin A Injection into the Lacrimal Glands: Application to Drug Efficacy Studies. J. Vis. Exp. (2020)

In this video, we demonstrate the inoculation of Concanavalin A, a lymphocyte mitogen, into the inferior lacrimal gland of the eye of a rabbit to induce dry eye disease. Ultrasound or sonogram images are used to guide the precise location of the injection site and also to confirm the success of inoculation.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Animals and Housing Acquire New Zealand White (NZW) rabbits weighing 2-3 kg. House the rabbits singly in cages with strict temperature (65 ± 5 °F) and humidity (45 ± 5%) control. Lighting should have a 12 h on/off cycle. Provide unlimited access to water and standard rabbit chow. Eliminate dietary enri…

Representative Results

Figure 1: Preparation of rabbit for concanavalin A injections. (A) Small shears are used to remove fur, allowing easier visualization of landmarks to identify the orbital superior lacrimal gland. (B) Nair is used to remove hair that remains after shearing. <str…

Divulgaciones

The authors have nothing to disclose.

Materials

Rabbit, New Zealand White Charles River Labs, Waltham, MA Research animals, 2-3 kg
Aceproinj (acepromazine) Henry Schein Animal Health, Dublin, OH NDC11695-0079-8 0.1ml/kg subcutaneously injection for rabbit sedation
Ultrasound probe VisualSonics Toronto, Ont MX 550 S Untrasonography-guide ConA injection for inferior lacrimal gland
Caliper Bausch and Lomb (Storz), Bridgewater, NJ E-2404 Caliper used to measure length of needle during ConA injection
Concanavalin A Sigma, St. Louis, MO C2010 Make 5mg/ml in PBS for injection into rabbit lacrimal glands
Isoflurane Henry Schein, Melville, NY 29405
Anesthesia vaporizer VetEquip, Pleasanton, CA Item #911103
26-gauge needles (5/8) Becton Dickinson and Company, Franklin Lakes, NJ 305115 Needles for injecting ConA into the lacrimal glands
27-gauge needles (5/8) Becton Dickinson and Company, Franklin Lakes, NJ 305921 Needles for injecting ConA into the lacrimal glands

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Ultrasound-Guided Concanavalin A Injection Into Inferior Lacrimal Gland: A Technique to Inoculate Inferior Lacrimal Gland With Concanavalin A to Induce Dry Eye Disease in Rabbit Model. J. Vis. Exp. (Pending Publication), e20911, doi: (2023).

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