Pig Bronchiolar Tissue Extraction: A Procedure to Dissect Bronchiolar Tissue Sections From Porcine Lungs
Published: April 30, 2023
Abstract
Source: Harrington, N. E. et al. Antibiotic Efficacy Testing in an Ex vivo Model of Pseudomonas aeruginosa and Staphylococcus aureus Biofilms in the Cystic Fibrosis Lung. J. Vis. Exp. (2021)
In this video, we describe a surgical procedure to extract bronchiolar tissues from harvested pig lung. The ex vivo bronchiolar tissue could be used to develop infection models for studying chronic lung infections.
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Dissection of ex vivo pig lung (EVPL) tissue Obtain lungs from the designated source as soon as possible after slaughter, ensuring they are kept cold by transporting to the laboratory in a domestic cool box. NOTE: Lungs closer to the day of slaughter show less bruising from storage, but tissue kept on cold storag…
Divulgaciones
The authors have nothing to disclose.
Materials
| 70% ethanol or similar for surface sterilizaton and flamin gof dissection equipment | |||
| Aluminum foil – pre-sterilised by autoclaving – to cover the chopping board on whcih you wil dissect lungs | |||
| Bunsen burner | |||
| Chopping board – we recommend a plastic board to allow for easy decontamination with alcohol. | |||
| Coolbox to transport lungs to lab | |||
| Dissection scissors in different sizes | |||
| Dulbecco's modified Eagle medium (DMEM) | |||
| Fisherbrand 2 mL reinforced tubes | Thermo Fisher | 15545809 | |
| Large pallet knife | |||
| Mounted razor blades | |||
| Plastic chopping board and aluminium foil to create a sterile and cleanable dissection surface | |||
| Roswell Park Memorial Institute (RPMI) 1640 medium | |||
| Selection of forceps (blunt tips recommended) | |||
| Suitable containers for disposing of contaminated sharps and pig ung tissue, according to your institution's health & safety policies. |
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Pig Bronchiolar Tissue Extraction: A Procedure to Dissect Bronchiolar Tissue Sections From Porcine Lungs. J. Vis. Exp. (Pending Publication), e20833, doi: (2023).