Intermittent Photic Stimulation in Rabbit Model: A Technique to Induce Epileptic Seizures and Simultaneously Record Multisystem Parameters
Published: April 30, 2023
Abstract
Source: Bosinski, C., et al. Multi-system Monitoring for Identification of Seizures, Arrhythmias and Apnea in Conscious Restrained Rabbits. J. Vis. Exp. (2021).
In this video, we show a procedure for simultaneous recording of video-EEG-ECG-capnography-oximetry data generated by inducing seizures in a rabbit model.
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Photic stimulation experiment Place a light source with a circular reflector 30 cm in front of the rabbit at eye level, with the flash intensity set to the maximum (16 candela). The light source is indicated by a white dot in Figure 1E. NOTE: A dimly lit room should be used to elicit the pho…
Representative Results
Figure 1: Rabbit connected to equipment. (A) Location of ECG electrodes, Left Arm is indicated by a yellow dot. Right Arm is indicated by a white dot. Left Leg is indicated by a red dot. Ground anterior to the right leg is indicated by a green dot. (B) Rabbit in restrainer with ECG and EEG electrodes attached. (C) Juvenile rabbit in a restrainer …
Divulgaciones
The authors have nothing to disclose.
Materials
| Computer | Dell | Optiplex 5040 | Acquisition computer |
| ECG Electrode | RhythmLink | RLSND116-2.5 | 13mm 35-degree bent (0.4 mm diameter) subdermal pin electrodes |
| EEG Electrode | RhythmLink | RLSP513 | 5-twist 13mm straight (0.4mm diameter) subdermal pin electrodes |
| EEGLAB (2020) | Swartz Center for Computational Neuroscience | Open Access | Can perform spectral analysis of EEG |
| Ethernet-to-ethernet adapter | Linksys | USB3G16 | Adapter for connecting the camera to the computer |
| Foam padding | Generic | N/A | Reduces pressure applied to the neck of small rabbits by the restrainer in order to prevent the adverse cardiorespiratory effects of neck compression |
| IR Light | Bosch | EX12LED-3BD-8W | Facilitates recordings in the dark |
| LabChart Pro (2019, Version 8.1.16) | ADInstruments | N/A | ECG Analysis |
| MATLAB (R2019b, Update 5) | MathWorks | N/A | Required to run EEGLAB |
| Microphone | Sony Stereo | ECM-D570P | Recording of audible manifestions of seizures |
| Micropore Medical Tape, Paper, White | 3M | 1530-1 | Used to secure wires and create ear splint |
| Natus NeuroWorks | Natus | LC101-8 | Acquisition and review software |
| Photic Stimulator | Grass | PS22 | Stimulator to control frequency, delay, duration, intensity of the light pulses |
| Plastic wire organizer / bundler | 12Vwire.com | LM-12-100-BLK | Bundle wires to cut down on noise |
| PS 22 Photic Stimulator | Grass Instruments | BZA641035 | Strobe light with adjustable flash frequency, delay, and intensity |
| PVC pipe | Generic | N/A | Prevents small rabbits from kicking their hind legs and causing spinal injury |
| Quantum Amplifier | Natus | 13926 | Amplifier / digitizer |
| Quantum HeadBox Amplifier | Natus | 22134 | 64-pin breakout box |
| Rabbit Restrainer | Plas-Labs | 501-TC | Various size rabbit restrainers are available. 6" x 18" x 6" in this study. |
| Rubber pad (booster) | Generic | N/A | Raises small rabbits up in the restrainer to prevent neck compression |
| SpO2 ear clip | NONIN | 61000 | PureSAT/SpO2 |
| SpO2 sensor adapter | NONIN | 13931 | XPOD PureSAT/SpO2 |
| SRG-X120 1080p PTZ Camera with HDMI, IP & 3G-SDI Output | Sony | SRG-X120 | Impela Camera |
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Intermittent Photic Stimulation in Rabbit Model: A Technique to Induce Epileptic Seizures and Simultaneously Record Multisystem Parameters. J. Vis. Exp. (Pending Publication), e20824, doi: (2023).