Tracer Molecule Delivery in the Cisterna Magna: A Method to Deliver Fluorescent Tracer Molecules via Direct Cannulation of the Cisterna Magna in Pig
Published: April 30, 2023
Abstract
Source: Bèchet, N. B., et al. Direct Cannula Implantation in the Cisterna Magna of Pigs. J. Vis. Exp. (2021).
This video describes the protocol for tracer molecule delivery via direct cannulation of the cisterna magna in a pig brain. This method helps visualize the distribution of cerebrospinal fluid in the glymphatic system.
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Cannulation and injection NOTE: This step requires at least two people and is carried out with the animal's head elevated and neck flexed. Begin with an anesthetized pig, pre-operated with surgically accessible cisterna magna. Ensure that one person elevates and flexes the head …
Divulgaciones
The authors have nothing to disclose.
Materials
| 18G needle | Mediq | ||
| 1ml Syringe | FischerSci | 15849152 | |
| 20G cannula | Mediq | NA | |
| 22G cannula | Mediq | NA | |
| Bovine serum albumin Alexa-Fluor 647 Conjugate | ThermoFischer | A34785 | 2 vials (10mg) |
| CaCl2 | Sigmaaldrich | C1016 | |
| Dental cement | Agnthos | 7508 | |
| Glucose | Sigmaaldrich | G8270 | |
| Insta-Set CA Accelerator | BSI-Inc | BSI-151 | |
| IV line TAP, 3-WAYS with 10cm extension | Bbraun | NA | |
| KCl | Sigmaaldrich | P9333 | |
| Marker pen | NA | NA | |
| MgCl2 | Sigmaaldrich | M8266 | |
| MilliQ water | NA | NA | |
| NaCL | Sigmaaldrich | S7653 | |
| NaH2PO4 | Sigmaaldrich | S8282 | |
| NaHCO3 | Sigmaaldrich | S5761 | |
| Saline | Mediq | NA | |
| Self-retaining retractors | NA | NA | |
| Superglue | NA | NA | |
| Surgical curved scissors | NA | NA | |
| Surgical towel clamps | NA | NA |
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Tracer Molecule Delivery in the Cisterna Magna: A Method to Deliver Fluorescent Tracer Molecules via Direct Cannulation of the Cisterna Magna in Pig. J. Vis. Exp. (Pending Publication), e20822, doi: (2023).