Bacterial Endophthalmitis Mouse Model: A Method for Generating Bacterial Endophthalmitis via Intravitreal Injection of Bacterial Suspension into Eye of Mouse
Published: April 30, 2023
Abstract
Source: Mursalin, M. H. et al., Intravitreal Injection and Quantitation of Infection Parameters in a Mouse Model of Bacterial Endophthalmitis. J. Vis. Exp. (2021).
This video describes a method for generating a mouse model that mimics bacterial endophthalmitis – an intraocular infection resulting from a bacterial invasion into the eye’s vitreous cavity – via intravitreal injection of a bacterial suspension into the eye. This mouse model helps study the disease mechanism and evaluate therapeutics for it.
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Sterile glass needles Turn On the needle pipette puller. Adjust the Heater knob until the display shows 12.6. Open the door and manually feed the 5 μL capillary tube through the upper clamp and heater filament until half of the tubing extends below the filame…
Representative Results
Figure 1: Making beveled glass needles. Glass needles were made from disposable microcapillary pipettes using a needle/pipette puller and a micropipette beveler. (A) Clamped glass capillary tube in the needle/pipette puller. (B,C) Creation of glass needles using the desired voltage. (D) Beveling the glass micropipettes. (E,F…
Divulgaciones
The authors have nothing to disclose.
Materials
| Cell Microinjector | MicroData Instrument, Inc. | PM2000 | NA |
| Microcapillary Pipets 5 Microliters | Kimble | 71900-5 | NA |
| Micro-Pipette Beveler | Sutter Instrument Co. | BV-10 | NA |
| Microscope OPMI Lumera | Zeiss | NA | |
| Reverse action forceps | Katena | K5-8228 | NA |
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Bacterial Endophthalmitis Mouse Model: A Method for Generating Bacterial Endophthalmitis via Intravitreal Injection of Bacterial Suspension into Eye of Mouse. J. Vis. Exp. (Pending Publication), e20816, doi: (2023).