Intravital Imaging: A Method for Cellular Level Observation of the Mouse Pancreas Through a Stabilized Imaging Window
Published: April 30, 2023
Abstract
Source: Park, I. and Kim, P. Stabilized Longitudinal In Vivo Cellular-Level Visualization of the Pancreas in a Murine Model with a Pancreatic Intravital Imaging Window. J. Vis. Exp. (2021).
In this video, we describe the intravital imaging of a mouse pancreas through a stabilized window for long-term visualization of cellular dynamics.
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Intravital imaging Turn on the intravital microscope including the laser power. Turn on the heating pad and set the homeothermic regulation to 37 °C. NOTE: Alternatively, use a passive heating pad or lamp with frequent control if there is no homeothermic regulation. Take …
Representative Results
Figure 1: Photograph of the implementation of the pancreatic intravital imaging window. A pancreatic intravital imaging window is implanted in the mouse in the XYZ translational stage and the imaging chamber holder attached to the tilting mount is connected to the pancreatic imaging window. Copyright 2020 Korean Diabetes Association from Diabetes Metab J. 2020 44:1:193-198. Reprinted with pe…
Divulgaciones
The authors have nothing to disclose.
Materials
| Alexa Fluor 647 Succinimidyl Esters (NHS esters) | Invitrogen | A20006 | Fluorescent probe for conjugate with antibody |
| BD Intramedic polyethylene tubing | BD Biosciences | 427401 | PE10 catheter for connection with needle |
| C57BL/6N | OrientBio | C57BL/6N | C57BL/6N |
| Cover glasses circular | Marienfeld | 0111520 | Cover glass for pancreatic imaging window |
| FITC Dextran 2MDa | Merck (Former Sigma Aldrich) | FD200S | For vessel identification |
| IMARIS 8.1 | Bitplane | IMARIS | Image processing |
| Intravital Microscopy | IVIM tech | IVM-C | Intravital Microscopy |
| MIP-GFP | The Jackson Laboratory | 006864 | B6.Cg-Tg(Ins1-EGFP)1Hara/J |
| Pancreatic imaging window | Geumto Engineering | Custom order | Pancreatic imaging window – custom order |
| Physiosuite | Kent Scientific | PS-02 | Homeothermic temperature controller |
| TMR Dextran 65-85kDa | Merck (Former Sigma Aldrich) | T1162 | For vessel identification |
| Window holder | Geumto Engineering | Custom order | Window holder – custom order |
| Zoletil | Virbac | Zoletil 100 | Anesthetic agent |
| Rompun | Bayer | Rompun | Anesthetic agent |
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Citar este artículo
Intravital Imaging: A Method for Cellular Level Observation of the Mouse Pancreas Through a Stabilized Imaging Window. J. Vis. Exp. (Pending Publication), e20797, doi: (2023).