Catheter Based Intravenous Injection of Experimental Agents: A Technique for Delivering Experimental Agents Intravenously Through Jugular Vein in Mouse Model
Published: April 30, 2023
Abstract
Source: Bornert, A. et al. In Vivo Two-photon Imaging of Megakaryocytes and Proplatelets in the Mouse Skull Bone Marrow. J. Vis. Exp. (2021)
In this video, we describe a method for implanting a catheter into the jugular vein of a mouse model through which experimental agents such as drugs and tracer dyes can be delivered intravenously in vivo. Such an arrangement when connected to other systems can also be used for monitoring hemodynamics and blood sampling.
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Preparation of mice and insertion of a catheter in the jugular vein NOTE: Here, male or female, 5-7-week-old mTmG reporter mice were used (B6.129(Cg) Gt(ROSA)26Sortm4(ACTBtdTomato,-EGFP)Luo10) crossed with Pf4-cre mice, allowing intense green fluorescence labeling in megakaryocyte…
Representative Results
Figure 1: Insertion of the catheter. (A) An incision is made to expose the jugular vein and the top of the pectoral muscle. (B) The catheter filled with saline is inserted in the jugular vein by passing through the muscle, creating a compression point. (C) The mouse is carefully turned so that it lies ventral surface downward wit…
Divulgaciones
The authors have nothing to disclose.
Materials
| Histoacryl 5 x 0, 5 mL | Braun | 1050052 | Injectable solution of surgical glue |
| Imalgene/Ketamine 1000 fl/10 mL | Boehring | 3661103003199.00 | Eye protection |
| Rompun Xylazine 2% fl/25 mL | Bayer | 4007221032311.00 | |
| Surflo IV catheter – Blue 22 G | Terumo | SR-OX2225C1 |
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Catheter Based Intravenous Injection of Experimental Agents: A Technique for Delivering Experimental Agents Intravenously Through Jugular Vein in Mouse Model. J. Vis. Exp. (Pending Publication), e20794, doi: (2023).