High-frequency Ultrasonography Based Early Pregnancy Characterization: A Technique to Study Embryo Implantation and Pregnancy Progression in Pregnant Murine Model
Published: April 30, 2023
Abstract
Source: Peavey, M. C. et al. A Novel Use of Three-dimensional High-frequency Ultrasonography for Early Pregnancy Characterization in the Mouse. J. Vis. Exp. (2017)
In this video, we demonstrate high-frequency ultrasonography to image embryo implantation sites in a pregnant murine model. This imaging technique successfully detects viable pregnancy and characterizes embryo-implantation sites during early murine pregnancy.
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Imaging the Pregnant Mouse Place mouse supine on the monitoring platform. The mouse continuously receives isoflurane anesthetic (between 1.5–2.5%) and oxygen (1 L/min) via the nose cone. Gently tape all paws to heart rate monitoring pads on the platform. Apply 1–2 mL of ultrasound transmissi…
Representative Results
Figure 1: High-frequency ultrasound detection of implantation site development and monitoring of fetal growth throughout gestation. As shown in Figure 1A, on 5.5 d.p.c. the lighter hyperechoic decidualized endometrium (D) allows the number of implantation sites and spacing to be quantified. As shown in Figure 1B, th…
Divulgaciones
The authors have nothing to disclose.
Materials
| VisualSonics Vevo 2100 Ultrasound Imaging Platform/ Machine | VisualSonics, inc. | VS-11945 | |
| Vevo Imaging Station | VisualSonics, inc. | SA-11982 | |
| Aquasonic 100 Ultrasound Transmission Gel | Parker | #SKU PLI 01-08 | |
| Isoflurane (IsoThesia) 100mL bottle | Henry Shein | #29404 | |
| PuraLubenAnimal Ophthalmic Ointment | Dechra | #12920060 |
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High-frequency Ultrasonography Based Early Pregnancy Characterization: A Technique to Study Embryo Implantation and Pregnancy Progression in Pregnant Murine Model. J. Vis. Exp. (Pending Publication), e20744, doi: (2023).