Brain Metastasis Mouse Model: A Method to Generate Brain Metastasis Mouse Model by Intracarotid Cancer Cell Injection
Published: April 30, 2023
Abstract
Source: Zhang, C. et al. Intracarotid Cancer Cell Injection to Produce Mouse Models of Brain Metastasis. J. Vis. Exp. (2017)
This video demonstrates the establishment of a brain metastasis mouse model by intracarotid injection of fluorescently labeled cancer cells. The mouse model can be used to study the effects of novel therapeutics and the underlying physiological mechanisms.
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Prepare Mice for Tumor Cell Injection Anesthetize a mouse by intraperitoneal (i.p.) injection of ketamine/xylazine cocktail (ketamine 100 mg/kg, xylazine 10 mg/kg). Confirm complete anesthesia by pinching the animal's feet and observe lack of response. Place the mouse on a glass p…
Divulgaciones
The authors have nothing to disclose.
Materials
| Ketamine hydrochloride/xylazine hydrochloride solution | Sigma-Aldrich | K113 | |
| Routine Stereomicroscope Leica M50 | Leica | M50 | The microscope is modular and highly configurable to fit particular space requirements |
| Surgical disposable scapel | Integra Miltex | 4-410 | To make skin incisions |
| Tissue Forceps – 1 x 2 Teeth | Fine Science Tools | 11021-12 | To bluntly dissect muscles |
| Dumont #5 – Mirror Finish Forceps | Fine Science Tools | 11252-23 | To separate and prepare the carotid artery for injection |
| Spring Scissors | Fine Science Tools | 15025-10 | To cut sutures |
| EZ Clip Kit | Stoelting | 59020 | For wound closure |
| BD Insulin Syringe | Becton Dickinson | 328438 | For cell injection |
| IVIS Spectrum In Vivo Imaging System | PerkinElmer | 124262 |
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Brain Metastasis Mouse Model: A Method to Generate Brain Metastasis Mouse Model by Intracarotid Cancer Cell Injection. J. Vis. Exp. (Pending Publication), e20688, doi: (2023).