Focus Ultrasound Based Microbubble Mediated Blood-Brain Barrier Opening: A Technique to Create Localized Transient Openings in Blood-Brain Barrier of Mouse by Sonoporation
Published: April 30, 2023
Abstract
Source: Haumann, R. et al. A High-Throughput Image-Guided Stereotactic Neuronavigation and Focused Ultrasound System for Blood-Brain Barrier Opening in Rodents. J. Vis. Exp. (2020)
This video describes a method of temporarily disrupting the blood-brain barrier (BBB) by creating transient openings using microbubble-mediated focused ultrasound (FUS). These transient local BBB openings can eventually be used to deliver larger-sized drug molecules directly into the brain parenchyma.
Protocol
All procedures involving human participants have been performed in compliance with the institutional, national and international guidelines for human welfare and have been reviewed by the local institutional review board. 1. Focused Ultrasound System NOTE: The described setup is an in-house built BBB disruption system based on commercially available components and includes a 3D-printed custom-made cone and deta…
Representative Results
Figure 1: Focused ultrasound setup. (A) Schematic representation of the focused ultrasound setup. (B) Picture of the focused ultrasound setup. The system consists of a top-down mounted transducer on a 1D linear stage over a second 2D stage for automatic 3D positioning. The transducer is built in a water-filled beam-cone, closed at the bottom with an acoustically …
Divulgaciones
The authors have nothing to disclose.
Materials
| 1 mL luer-lock syringe | Becton Dickinson | 309628 | Plastipak |
| 19 G needle | Terumo Agani | 8AN1938R1 | |
| 23 G needle | Terumo Agani | 8AN2316R1 | |
| 3M Transpore surgical tape | Science applied to life | 7000032707 | Or similar |
| Arbitrary waveform generator | Siglent | SDG1025, 25 mHz, 125 MSa/s | |
| Automated stereotact | In-house built | Stereotact with all elements were in-house built | |
| Bruker In-Vivo Xtreme | Bruker | Includes software | |
| Buffered NaCl solution | B. Braun Melsungen AG | 220/12257974/110 | |
| Buprenorfine hydrochloride | Indivior UK limitd | 0.324 mg | |
| Cage enrichment: paper-pulp smart home | Bio services | ||
| Carbon filter | Bickford | NC0111395 | Omnicon f/air |
| Ceramic spoon | |||
| Cotton swabs | |||
| D-luciferin, potassium salt | Gold Biotechnology | LUCK-1 | |
| Ethanol | VUmc pharmacy | 70% | |
| Evans Blue | Sigma Aldrich | E2129 | |
| Fresenius NaCl 0.9% | Fresenius Kabi | Nacl 0.9 %, 1000 mL | |
| Histoacryl | Braun Surgical | Histoacryl 0.5 mL | |
| Hydrophone | Precision Acoustics | ||
| Insulin syringe | Becton Dickinson | 324825/324826 | 0.5 mL and 0.3 mL |
| Isoflurane | TEVA Pharmachemie BV | 8711218013196 | 250 mL |
| Ketamine | Alfasan | 10 %, 10 mL | |
| Mouse food: Teklad global 18% protein rodent diet | Envigo | 2918-11416M | |
| Neoflon catheter | Becton Dickinson | 391349 | 26 GA 0.6 x 19 mm |
| Oscilloscope | Keysight technologies | Infiniivision DSOX024A | |
| Plastic tubes | Greiner bio-one | 210261 | 50 mL |
| Power amplifier | Electronics & Innovation Ltd | 210L | Model 210L |
| Preamplifier DC Coupler | Precision Acoustics | Serial number: DCPS94 | |
| Scissors | Sigma Aldrich | S3146-1EA | Or similar |
| Sedazine | AST Farma | 2% | |
| SonoVue microbubbles | Bracco | 8 µl/mL | |
| Sterile water | Fresenius Kabi | 1000 mL | |
| Syringe | Various syringes can be used | ||
| Temgesic | Indivior UK limitd | 0.3 mg/mL | |
| Transducer | Precision Acoustics | 1 mhz | |
| Tweezers | Sigma Aldrich | F4142-1EA | Or similar |
| Ultrasound gel | Parker Laboratories Inc. | 01-02 | Aquasonic 100 |
| Vidisic gel | Bausch + Lomb | 10 g |
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Focus Ultrasound Based Microbubble Mediated Blood-Brain Barrier Opening: A Technique to Create Localized Transient Openings in Blood-Brain Barrier of Mouse by Sonoporation. J. Vis. Exp. (Pending Publication), e20684, doi: (2023).