Intracranial Orthotopic Model: A Procedure to Implant Cancer Cells in Mouse Brain
Published: April 30, 2023
Abstract
Source: Geisler, J. A. et al. Modeling Brain Metastases Through Intracranial Injection and Magnetic Resonance Imaging. J. Vis. Exp. (2020)
This video demonstrates implantation of tumor cells in the mouse brain by performing intracranial injection.
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Injecting Cancer Cells into the Brain Place an anesthetized mouse with an exposed calvarial window on the stereotatic frame. Attach an automatic injector unit to the stereotactic apparatus (Figure 1). Pull up >2 µL of cells thoroughly resuspended in 1x DPBS in a clean Hamilt…
Representative Results
Figure 1: Stereotactic and anesthesia systems. Stereotactic stand with automated syringe pump and mouse elevating box. The box is an inverted pipette tip box containing hand warmers used to elevate the mouse to the appropriate height and maintain body temperature. Inset shows the orientation of the Hamilton syringe in the automated injection apparatus.
Divulgaciones
The authors have nothing to disclose.
Materials
| Bone Wax | Surgical Specialities | 903 | Sterile and malleable beeswax and isopropyl palmitate |
| Cotton tip applicators | Puritan | 25-806 10WC | Sterile long stemmed cotton tip applicators |
| Suture | Covidien | SP5686G | 45 cm swedged 5-0 monofilament polypropylene suture |
| Skin Glue | Vetbond | 1469SB | Skin safe wounds adhesive |
| Stereotaxic Unit | |||
| Mouse Gas Anesthesia Head Holder | Kopf | Model 923-B | Mouth bar with teeth hole and nosecone |
| Non-Rupture Ear Bars | Kopf | Model 922 | Ear bars suitable for mouse applications |
| Stereotaxic Instrument | Kopf | Model 940 | Base plate, frame and linear scale assembly with digital readout monitor |
| Injector | |||
| Injector Needle and syringe | Hamilton | 80366 | 26 gauge needle, 51 mm needle length and 10 μL volume syringe |
| Legato 130A automated Syringe Pump | KD Scientific | P/N: 788130 | Programmable touch screen base with automated injector |
| Anesthesia Machine | |||
| SomnoSuite Low-Flow Digital Vaporizer | Kent Scientific | SS-01 | Digital anesthesia machine |
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Intracranial Orthotopic Model: A Procedure to Implant Cancer Cells in Mouse Brain. J. Vis. Exp. (Pending Publication), e20610, doi: (2023).