Modeling Orthotopic Bladder Tumor in Mouse Model: A Procedure for Intravesical Administration of Cancer Cells into Murine Bladder

Published: April 30, 2023

Abstract

Source: Tham, S. M. et al. A Murine Orthotopic Bladder Tumor Model and Tumor Detection System. J. Vis. Exp. (2017)

In this video, we describe the intravesical procedure of delivering cancer cells into the bladder of a mouse model. This procedure helps generate an orthotopic bladder tumor model that can be used to develop therapeutic strategies.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Implanting the Tumor NOTE: Each mouse is implanted with 1 x 105 MB49-PSA cells in 50 µL of DMEM blank media in the bladder. Due to the dead space in the catheter, always prepare extra volume (at least 100 µL extra per mouse). An alternative approach would be to us…

Divulgaciones

The authors have nothing to disclose.

Materials

MB49-PSA cells  Ref (Wu QH, 2004)
Dulbecco's Modified Eagle's
Medium (DMEM) media
Biowest  L0102
Fetal Bovine Serum (FBS) South American  Biowest  S1810
Fetal Bovine Serum (FBS) South American, Premium  Biowest  S181B
Fetal Bovine Serum (FBS) HyClone  SH30088.03
L-glutamine Biowest X0550   Biowest  X0550
Penicillin-Streptomycin  Biowest  L0022
Hygromycin B  Invitrogen  10687-010
C57BL/6J female mice  In Vivos  4-6 wk old
Anesthesia (75 mg/kg Ketamine and 1 mg/kg Medetomidine)    Local pharmacy 
Reversal drug (1 mg/kg Atipamezole)  Local pharmacy 
Ear punch  Electron Microscopy Sciences  72893-01
Hartmann's solution or Compound sodium lactate  B Braun
Ophthalmic ointment – Duratears sterile ocular lubricant ointment  Alcon
Heat pack – HotHands handwarmers  Heatmax Inc 
Introcan Certo IV catheter   B Braun  4251300 24 G x 3/4″
Aquagel Lubricating jelly   Local pharmacy
Poly-L-lysine solution, 0.01% Sigma P4707

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Modeling Orthotopic Bladder Tumor in Mouse Model: A Procedure for Intravesical Administration of Cancer Cells into Murine Bladder. J. Vis. Exp. (Pending Publication), e20561, doi: (2023).

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