Modeling Orthotopic Bladder Tumor in Mouse Model: A Procedure for Intravesical Administration of Cancer Cells into Murine Bladder
Published: April 30, 2023
Abstract
Source: Tham, S. M. et al. A Murine Orthotopic Bladder Tumor Model and Tumor Detection System. J. Vis. Exp. (2017)
In this video, we describe the intravesical procedure of delivering cancer cells into the bladder of a mouse model. This procedure helps generate an orthotopic bladder tumor model that can be used to develop therapeutic strategies.
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Implanting the Tumor NOTE: Each mouse is implanted with 1 x 105 MB49-PSA cells in 50 µL of DMEM blank media in the bladder. Due to the dead space in the catheter, always prepare extra volume (at least 100 µL extra per mouse). An alternative approach would be to us…
Divulgaciones
The authors have nothing to disclose.
Materials
| MB49-PSA cells | Ref (Wu QH, 2004) | ||
| Dulbecco's Modified Eagle's Medium (DMEM) media |
Biowest | L0102 | |
| Fetal Bovine Serum (FBS) South American | Biowest | S1810 | |
| Fetal Bovine Serum (FBS) South American, Premium | Biowest | S181B | |
| Fetal Bovine Serum (FBS) | HyClone | SH30088.03 | |
| L-glutamine Biowest X0550 | Biowest | X0550 | |
| Penicillin-Streptomycin | Biowest | L0022 | |
| Hygromycin B | Invitrogen | 10687-010 | |
| C57BL/6J female mice | In Vivos | 4-6 wk old | |
| Anesthesia (75 mg/kg Ketamine and 1 mg/kg Medetomidine) | Local pharmacy | ||
| Reversal drug (1 mg/kg Atipamezole) | Local pharmacy | ||
| Ear punch | Electron Microscopy Sciences | 72893-01 | |
| Hartmann's solution or Compound sodium lactate | B Braun | ||
| Ophthalmic ointment – Duratears sterile ocular lubricant ointment | Alcon | ||
| Heat pack – HotHands handwarmers | Heatmax Inc | ||
| Introcan Certo IV catheter | B Braun | 4251300 | 24 G x 3/4″ |
| Aquagel Lubricating jelly | Local pharmacy | ||
| Poly-L-lysine solution, 0.01% | Sigma | P4707 |
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Modeling Orthotopic Bladder Tumor in Mouse Model: A Procedure for Intravesical Administration of Cancer Cells into Murine Bladder. J. Vis. Exp. (Pending Publication), e20561, doi: (2023).