Unilateral Ureteral Obstruction Murine Model: A Surgical Procedure to Obstruct the Urinary Flow from a Kidney in Mouse Models
Published: April 30, 2023
Abstract
Source: Yanai, K. et al. Quantitative real-time PCR evaluation of microRNA expressions in mouse kidney with unilateral ureteral obstruction. J. Vis. Exp. (2020)
This video demonstrates the procedure to generate a unilateral ureteral obstruction mouse model. The procedure involves cutting one of the ureters of the mouse to obstruct urine flow.
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. The UUO Surgery Prepare the following items: isoflurane, cork sheet, depilatory cream, laboratory wipes, Petri dish with PBS, 4-0 silk, 4-0 nylon, a 2.5 mL syringe, cotton swabs, tweezers, surgical scissors, and 8-week old C57BL/6 male mice. Anesthetize a mouse with 1.5% isoflurane and maintai…
Divulgaciones
The authors have nothing to disclose.
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Unilateral Ureteral Obstruction Murine Model: A Surgical Procedure to Obstruct the Urinary Flow from a Kidney in Mouse Models. J. Vis. Exp. (Pending Publication), e20555, doi: (2023).