Spinal Cord Extraction: A Protocol to Isolate Murine Spinal Cord for In Vitro Studies

Published: April 30, 2023

Abstract

Source: Na'ara, S. et al. In Vitro Modeling of Cancerous Neural Invasion: The Dorsal Root Ganglion Model. J. Vis. Exp. (2016)

In this video, we describe a step-by-step protocol to isolate spinal cord from a mouse model for in vitro studies.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Harvesting the Spinal Cord Euthanize the mouse using a CO2 chamber. Avoid cervical dislocation as it might cause damage to the ganglion roots due to shear forces. From here on, perform all steps under sterile conditions. Soak the animal with 70% ethanol. This step is important for sterilization…

Divulgaciones

The authors have nothing to disclose.

Materials

Forceps  Sigma-Aldrich  F4142
Surgical blade  Sigma-Aldrich  Z309036
Scissors  Sigma-Aldrich  S3271
35 mm Petri dishes, glass bottom  de groot  60-627860
70% ethanol  Sigma
Cold PBS  Biological industries  02-023-1A

Tags

Play Video

Citar este artículo
Spinal Cord Extraction: A Protocol to Isolate Murine Spinal Cord for In Vitro Studies. J. Vis. Exp. (Pending Publication), e20489, doi: (2023).

View Video