Snap Freezing of Muscle Tissue for Sectioning: A Protocol to Obtain Ultrathin Sections of Rapidly Frozen Murine Muscle Tissue
Published: April 30, 2023
Abstract
Source: Bonetto, A. et al. The Colon-26 Carcinoma Tumor-bearing Mouse as a Model for the Study of Cancer Cachexia. J. Vis. Exp. (2016)
This video describes the procedure for snap freezing muscle tissue and obtaining its thin sections. The technique can help study cancer-related cachexia in colon cancer mouse models.
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Muscle Freezing and Mounting Immerse the bottom half of a small plastic beaker (50 mL) containing isopentane into liquid nitrogen. The isopentane is ready to use when it becomes slightly viscous and forms a solid white laminate lining the inside of the beaker (temperature: -160 °C). NOTE: Al…
Divulgaciones
The authors have nothing to disclose.
Materials
| OCT | Tissue-Tek | 4583 | |
| Cryostat | Leica | CM1850 | |
| Cork disks | Electron Microscopy Sciences | 63305 | |
| Superfrost plus glass slides | VWR | 48311-703 |
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Snap Freezing of Muscle Tissue for Sectioning: A Protocol to Obtain Ultrathin Sections of Rapidly Frozen Murine Muscle Tissue. J. Vis. Exp. (Pending Publication), e20423, doi: (2023).