Negative Staining of Purified Exosomes: A Procedure to Visualize Exosome Morphology Using Transmission Electron Microscopy
Published: April 30, 2023
Abstract
Source: Jung, M. K. et al. Sample Preparation and Imaging of Exosomes by Transmission Electron Microscopy. J. Vis. Exp. (2018)
This video describes a procedure for negative staining of purified exosomes. The negatively stained exosomes are imaged using transmission electron microscopy. This technique helps visualize the morphology of exosomes.
Protocol
1. Negative Staining Glow-discharge the thin formvar/carbon film-coated 200 mesh copper EM grids for 1 min by glow discharger. Fix purified exosomes with 1 mL of 2% paraformaldehyde (PFA) for 5 min. NOTE: Paraformaldehyde fumes are toxic. All work should be done in a ventilated fume hood. Load 5-7 µL exosome suspension solution on the grid and incubate for 1 min. If the concentration of exosome is too high, dilute the concentr…
Divulgaciones
The authors have nothing to disclose.
Materials
| Uranyl acetate | EMS | 22400 | Hazardous chemical |
| Transmission Electron Microscopy | JEOL | JEM2200FS | |
| Glow discharger | JEOL | JFC1100E | |
| Paraformaldehyde | EMS | 19210 | |
| Formvar carbon coated Copper Grid (200 mesh) |
EMS | FCF200-CU | |
| Formvar carbon coated Nickel Grid (200 mesh) |
EMS | FCF200-NI |
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Negative Staining of Purified Exosomes: A Procedure to Visualize Exosome Morphology Using Transmission Electron Microscopy. J. Vis. Exp. (Pending Publication), e20420, doi: (2023).