FFPE Tumor Tissue Macrodissection: A Technique to Obtain Specific Tumorous Tissue from Unstained Specimens
Published: April 30, 2023
Abstract
Source: Sugimoto, K. et al. Genome-Wide Analysis of DNA Methylation in Gastrointestinal Cancer. J. Vis. Exp. (2020)
This video describes a method for the manual macrodissection of cancer tissue from unstained tissue specimens to obtain a specific cancer tissue. The tissue section can be processed further to obtain DNA for genome-wide studies on gastrointestinal cancers.
Protocol
1. Scraping the Slides Prepare digestion/lysis buffer with 650 mL of diethylpyrocarbonate (DEPC)-treated water, 100 mL of ethylenediaminetetraacetic acid (EDTA), 50 ml of Tris hydrochloride (Tris-HCL) 2 M pH 8.8, and 200 mL of 10% sodium dodecyl sulfate (SDS) (see Table of Materials). Fill a 1.5 mL single-use polypropylene tube with 80 µL of digestion/lysis buffer (see Table of Materials). Put a clean pipette tip into the buf…
Divulgaciones
The authors have nothing to disclose.
Materials
| 10% Sodium dodecyl sulfate (SDS) | Quality Biological | 351-032-721 EA | |
| DEPC-treated water | Quality Biological | 351-068-131 | |
| Ethylenediaminetetraacetic acid (EDTA) | Corning | 46-034-CL | |
| Tris hydrochloride (Tris-HCL) 2 M pH 8.8 | Quality Biological | 351-092-101 | |
| Proteinase K | New England Biolabs | P8107S | |
| Single-use polypropylene (Eppendorf) tube | Eppendorf | 24533495 |
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FFPE Tumor Tissue Macrodissection: A Technique to Obtain Specific Tumorous Tissue from Unstained Specimens. J. Vis. Exp. (Pending Publication), e20409, doi: (2023).