Sampling Prostate Cancer Tissue for Biobanking: An MRI-guided Targeted Technique to Remove Biopsy Punches from Fresh Tumor Tissue
Published: April 30, 2023
Abstract
Source: Heavey, S. et al. Use of Magnetic Resonance Imaging and Biopsy Data to Guide Sampling Procedures for Prostate Cancer Biobanking. J. Vis. Exp. (2019)
This video demonstrates an MRI data-based technique to target specific tumor-containing regions in prostate tissue for sampling. The collected biopsy specimens can be either stored for biobanking purposes or used for subsequent downstream analysis.
Protocol
All procedures involving human participants have been performed in compliance with the institutional, national, and international guidelines for human welfare and have been reviewed by the local institutional review board. 1. Prostate Slicing Apparatus Purchase the prostate slicing apparatus (Table of Materials). Alternatively, print a blade handle using a 3D printer. 2. Tumor Targeting <l…
Representative Results
Figure 1: Prostate slicing apparatus. (A) The walls are inserted perpendicular to the base, and gold pins are inserted into the base surrounding the prostate (prostate not pictured). (B) The replaceable parallel blades are inserted into the blade handle. (C) The blades pass between the gold pins in order to slice a 5 mm section of the prost…
Divulgaciones
The authors have nothing to disclose.
Materials
| 6 mm biopsy punch | Fisher Scientific | 13404607 | Disposable biopsy punches for removing 6 mm tissue samples |
| Black Ink | Leica Biosystems | 3801753 | Tissue marking & margin dye |
| Blue Ink | Leica Biosystems | 3801751 | Tissue marking & margin dye |
| Chainmail hand glove | Arco | 1456803 | Chainmail gloves to protect hand during slicing |
| Cork board | Fisher Scientific | 12396447 | Cork board for pinning prostate to following sampling procedure |
| Needles | SLS (Scientific Laboratory supplies) | SYR6112 | Sterile needles to use to pin tissue to cork board following sampling |
| Prostate slicing aparatus | Insitute of Cancer Research, London | NA – must be obtained under MTA | A kit containing the slicer handle, blades, spacer, base, walls and pins |
Citar este artículo
Sampling Prostate Cancer Tissue for Biobanking: An MRI-guided Targeted Technique to Remove Biopsy Punches from Fresh Tumor Tissue. J. Vis. Exp. (Pending Publication), e20392, doi: (2023).