Intra-uterine Cancer Cell Injection: A Technique to Deliver Cancer Cells for Establishing Intra-peritoneal Ovarian Cancer Mouse Model
Published: April 30, 2023
Abstract
Source: Sumi, N. J. et al. Murine Model for Non-invasive Imaging to Detect and Monitor Ovarian Cancer Recurrence. J. Vis. Exp. (2014)
In this video, we demonstrate intra-uterine injection of ovarian cancer cells in a mouse model. This orthotopic murine model helps us to non-invasively monitor tumor growth and evaluate the efficacy of new therapies for the prevention and treatment of recurrent ovarian cancer.
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Intra-uterine Injection of Human Ovarian Cancer Cells in Athymic Nude Mice NOTE: The following procedure requires assistance from a second person. In addition, since this is survival surgery, use sterile surgical instruments. This survival surgery procedure should take approximately 1…
Representative Results
Figure 1: Intra-uterine injection of ovarian cancer cells. (A) anesthesia is continuously administered via a nose cone; (B-E) skin is incised to locate and clamp the uterine horn; (F) cancer cells are injected slowly into the lumen of the uterus.
Divulgaciones
The authors have nothing to disclose.
Materials
| Alcohol pads | Fischer Scientific | 06-669-62 | |
| Athymic nude mice | Harlan | ||
| Hemostat | VWR | 82027-422 | |
| Ibuprofen | Walgreens | Children's Ibuprofen 100 (100 mg/5 ml) | |
| Isoflurane | Butler Schein | NDC 11695-6776-1 | |
| Surgical forceps | VWR | 82027-386 | |
| Surgical scissors | VWR | 82027-584 | |
| Synthetic absorbable suture | Covidien | SL-636 | |
| Tissue adhesive | Vetbond | 1469SB | |
| 1 ml syringe | Becton Dickinson | 309602 | |
| 25 gauge needle | Becton Dickinson | 305122 | |
| Paclitaxel | Hospira, Inc. | NDC 61703-345-50 | |
| Puralube | Pharmaderm |
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Intra-uterine Cancer Cell Injection: A Technique to Deliver Cancer Cells for Establishing Intra-peritoneal Ovarian Cancer Mouse Model. J. Vis. Exp. (Pending Publication), e20380, doi: (2023).