Lung Instillation and Fixation: Introducing Liquid into the Trachea and Fixing Lungs to Measure the Fixed Lung Volume
Published: April 30, 2023
Abstract
Source: Limjunyawong, N. et al, Instillation and Fixation Methods Useful in Mouse Lung Cancer Research. J. Vis. Exp. (2015).
This video describes a simple and reliable method of introducing liquid into the trachea for lung instillation, fixation, and lung volume measurement.
Protocol
All procedures involving animals have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Lung Instillation Select a commercial one-inch long 20 g intravenous cannula to use for the intubation. Modify the catheter tip manually bending it to generate a slight curvature at the tip as illustrated in Figure 1. Anesthetize the mouse with a mixture of ketamine (100 mg/kg) and xylazin…
Representative Results
Figure 1: Intravenous catheter with slightly curved tip used for instillation. Figure 2: Sloped 3-ring binder used to support mice for the intubation. This binder set up to hold 3 mice. <p class="jove_conte…
Divulgaciones
The authors have nothing to disclose.
Materials
| Laboratory Balance | Ohaus | Adventurer Pro Model AV 313 | Other balances can be used if they have a range of 1-300 g |
| 20 g Luer intravenous catheter | Insylte | Several other possible vendors, e.g., Jelco Optiva | |
| 500 ml laboratory bottle | Various | Several other possible vendors |
Citar este artículo
Lung Instillation and Fixation: Introducing Liquid into the Trachea and Fixing Lungs to Measure the Fixed Lung Volume. J. Vis. Exp. (Pending Publication), e20251, doi: (2023).