Antibody Microarray: A Technique to Study the Protein Expression of miRNA Treated Lung Cancer Cells
Published: April 30, 2023
Abstract
Source: Hossian, A. et. al. Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis. J. Vis. Exp. (2019).
This video describes the technique to study the protein expression in miRNA transfected lung cancer cells by using antibody microarray to measure specific gene expression. In the featured protocol, we perform antibody microarray for the identification of the activity of a combinatorial miRNA treatment in halting cell cycle and angiogenesis.
Protocol
1. miR-143 and miR-506 transfection CAUTION: Use latex gloves, protective eyeglasses, and a laboratory coat while performing the described experiments. When required, use the biosafety cabinet with the cabinet fan on, without blocking the airways or disturbing the laminar airflow. Always set the protecting glass window to the appropriate height, as described by the manufacturer. Seed NSCLC A549 cells in a T25 cm2 flask/6/96 well plate in DMEM/F12K m…
Divulgaciones
The authors have nothing to disclose.
Materials
| A549 Non Small Cell Lung Cancer Cells | ATCC | ATCC CCL-185 | |
| Penicillin-streptomycin 10/10 | Antlanta Biologicals | B21210 | |
| Fetal Bovine Serum (FBS) | Fisher Scientific | 10438026 | |
| Antibody Array Assay Kit, 2 Reactions | Full Moon Bio | KAS02 | |
| Cell Cycle Antibody Array, 2 Slides | Full Moon Bio | ACC058 | |
| ScanArray Express | PerkinElmer | Step 2.33: Microarray analysis software | |
| HyClone Phosphate Buffered Saline (PBS) | Fisher Scientific | SH30256FS | |
| Thermo Scientific Pierce BCA Protein Assay | Thermo Scientific | PI23226 |
Citar este artículo
Antibody Microarray: A Technique to Study the Protein Expression of miRNA Treated Lung Cancer Cells. J. Vis. Exp. (Pending Publication), e20239, doi: (2023).