Intrabronchial Delivery: A Technique to Administer an Experimental Agent Selectively Into a Mouse Lung
Published: April 30, 2023
Abstract
Source: Liao, S. et. al. Direct Intrabronchial Administration to Improve the Selective Agent Deposition Within the Mouse Lung. J. Vis. Exp. (2019).
This video describes an intrabronchial approach to administer an experimental agent selectively into distal right and left lung. In the featured protocol, we will administer bleomycin intrabronchially into a mouse to obtain a better model for pulmonary fibrosis.
Protocol
All procedures involving animals have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Non-operative IB intubation and delivery approaches IB approach to selective lobar cannulation of the distal right lung After performing intratracheal (IT) cannulation (use the dominant hand to intubate the extended catheter into the trachea via the oral cavity), rotate the plastic board +3…
Representative Results
Figure 1: Approach for selective right/left lung lobar cannulation and administration. (A) To target the right lung, the plastic board is rotated +30°, improving ease of selectively engaging the right mainstem bronchus. The catheter is advanced (per distances proposed in Table 1) to selectively engage right …
Divulgaciones
The authors have nothing to disclose.
Materials
| 22 G shielded IV Catheter | BD | 381423 | |
| Evans blue dye | Sigma-Aldrich | E2129 | |
| LED-30W Fiber Optic Dual Gooseneck Lights Microscope Illuminator | AmScope | LED-30W | |
| Precisionglide needle, 18G x 1" | BD | 305195 | Beveled tip, 12 mm in length |
Citar este artículo
Intrabronchial Delivery: A Technique to Administer an Experimental Agent Selectively Into a Mouse Lung. J. Vis. Exp. (Pending Publication), e20233, doi: (2023).