Zebrafish Electrocardiography (ECG): A Minimally Invasive Assay to Evaluate Cardiac Function
Published: April 30, 2023
Abstract
Source: Zhao, Y., et al. In Vivo Surface Electrocardiography for Adult Zebrafish. J. Vis. Exp. (2019).
This video shows how to perform electrocardiography (ECG or EKG) measurements, a test which measures the electrical activity of the heart, on a zebrafish.
Protocol
This protocol is an excerpt from Zhao et al, In Vivo Surface Electrocardiography for Adult Zebrafish, J. Vis. Exp. (2019). All experiments in this study were conducted in accordance with the US National Institutes of Health Guide for the Care and Use of Laboratory Animals. All animal protocols in this study were approved by the UCLA Institutional Animal Care and Use Committee. 1. Anesthesia induction Prepare…
Representative Results
Figure 1: Minimally invasive in vivo ECG recording protocol. A schematic flow chart illustrates four critical action steps in conducting an in vivo ECG interrogation: induce anesthesia, place ECG lead electrodes, record ECG, and analyze the ECG recordings. Please click here to view a larger version of this figure. <img src="/fil…
Materials
| Culture dishes | Fisher Scientific | FB087571 | 100 mm x 20 mm |
| Dumont Forceps | Fine Sciense Tools | 11253-20 | 0.1 x 0.06 mm |
| FE136 Animal Bio Amp | AD Instruments | FE231 | |
| Iris Forceps | Fine Sciense Tools | 11064-07 | 0.6 x 0.5 mm |
| LabChart 8 Pro | AD Instruments | Software with ECG Module | |
| Needle electrodes for Animal Bio Amp | AD Instruments | MLA1213 | 29 gauge |
| Plastic Disposable Transfer Pipets | Fisher Scientific | 13-669-12 | 6 in., 1.2 mL |
| PowerLab 4/35 | AD Instruments | 4//35 | |
| Tricaine (Ethyl 3-aminobenzoate methanesulfonate) | Sigma | E10521-10G | MS-222 |
Citar este artículo
Zebrafish Electrocardiography (ECG): A Minimally Invasive Assay to Evaluate Cardiac Function. J. Vis. Exp. (Pending Publication), e20079, doi: (2023).